Population genetic variation analysis of bitter gourd wilt caused by Fusarium oxysporum f. sp. momordicae in China using inter simple sequence repeats (ISSR) molecular markers

Abstract Bitter gourd wilt caused by Fusarium oxysporum f. sp. momordicae (FOM) is a devastating crop disease in China. A total of 173 isolates characteristic typical with abundant microconidia and macroconidia on white or ruby colonies were obtained from diseased plant tissues. BLASTn analysis the rDNA-ITS showed 99% identity F. species. Among tested isolates, three infectious toward tower five pathogenic to bottle gourd. However, all bitter For genetic differences analysis, 40 ISSR primers screened 11 used for ISSR-PCR amplification. In total, 127 loci detected, which 76 polymorphic at rate 59.84%. POPGENE that Nei’s gene diversity index (H) Shannon’s information (I) 0.09 0.15, respectively, indicated was low. The coefficient differentiation (Gst = 0.33 > 0.15) mainly among populations. strength flow (Nm 1.01 1.0) weak, indicating population drift blocked some degree. dendrogram based markers nine geographical populations clustered into two groups threshold similarity 0.96. Shandong Henan Group I, while Guangdong, Hainan, Guangxi, Fujian, Jiangxi, Hubei constituted II. Results variation Hunan Guangxi had highest degree differentiation, lowest differentiation. Our findings enrich knowledge characteristics FOM goal developing effective disease-management programs resistance breeding programs.